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Imvu T3DE Crack

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A rapid, cell-based, radioactive immunoassay for the renal and hepatic elimination of drugs is described. The methods are based on mouse monoclonal antibodies raised against the anionic drug p-aminohippuric acid (PAH), which is conjugated to bovine serum albumin. The assay detects the metabolites of PAH in urine and blood by a competitive binding between the metabolite and drug with the antibody for a fixed number of radioactivity on the stationary phase. The method is validated with small molecules and is applied to a number of anionic compounds (caffeine, cephalexin, alprazolam, S-methyl PAH) and acidic drugs (diclofenac, ketoprofen, lansoprazole) using Caco-2 cell monolayers to predict their renal and hepatic clearance. The results obtained by the two methods are in good agreement and compared well with rat in vivo clearance data. The method is sensitive (0.5 ng/mL) and relatively fast (examples 1-2 hr) with high sample throughput (130 samples/hr). Specific analysis of unlabeled drug by direct competitive inhibition of labeled drug with a known amount of unlabeled drug is also feasible. The assay is amenable to automation and has high potential for application to large scale studies.For indispensable reporting on the coronavirus crisis, the election, and more, subscribe to the Mother Jones Daily newsletter.

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